ABSTRACT
Objective: To study the protective role of omega 3 fatty acids [omg 3 FAs] on the histomorphological changes in the height of the prostatic epithelium in rats induced by sleep deprivation
Study Design: Lab based randomized control trial
Place and Duration of Study: The study was conducted at Anatomy Department, Army Medical College, Rawalpindi, in collaboration with National Institute of Health [NIH], Rawalpindi for duration of one year, from Nov 2014 to Nov 2015
Material and Methods: Thirty male Sprague Dawley rats, 3-4 months of age with average weights of 200-300 grams [gm] were divided in three groups each having 10 rats. Group A served as control with standard lab diet and regular sleep -wake cycle. Group B was subjected to sleep deprivation of 16 hours followed by a sleep window of 8 hrs daily for 2 months and group C was administrated with omg 3 fatty acids [FAs] and was sleep deprived as group B for 2 months. At the end of the experimental period rats were anesthetized and their blood sample was drawn for hormonal assay. They were dissected and the prostate gland was removed and fixed in 10 percent formalin. Five micrometer [micro m] sections were obtained after tissue processing and stained with haematoxylin and eosin [HandE] for histological study
Results: Microscopic examination revealed that the epithelium of glandular acini was columnar in group A. Marked decrease in the height of cells was observed in group B whereas the epithelium was nearly cuboidal in group C
Conclusion: It was concluded that sleep deprivation had deleterious effects on the epithelium of the prostatic acini and that Omega 3 fatty acids had a protective effect on the epithelium of the prostatic acini
ABSTRACT
Objective: To evaluate the protective effect of zinc on histomorphometry of femur of Sprague Dawley rats under high salt diet
Study Design: Analytical randomized control trial
Place and Duration of Study: The studied was carried out at Islamic International Medical College, Rawalpindi, with the collaboration of National Institute of Health; Islamabad. The study spanned over a period of six months, from Sep 2015 to Mar 2016
Material and Methods: Forty five female Sprague Dawley rats, 10-12 weeks old were used in the study. The rats were randomly divided into 3 groups. Group-A rats were fed high salt diet [8% NaCl] and group B rats were given high salt diet augmented with zinc [50mg/kg/day] for eight weeks, however, the diet of control group was not modified. Rats were dissected and left femora were removed. Decalcification was performed. Tissue from proximal femur was obtained to study the trabecular structure and collagen staining while midshaft of femur was transversely sectioned to measure the medullary cavity diameter. Processing was done to obtain five micrometer [micro m] thick sections. Tissues were stained with haematoxylin and eosin [H and E] and Masson's Trichrome for histological parameters. Comparison was done amongst all groups
Results: Obvious histological variations were observed in experimental groups. These changes were of more severity in rats of group-A who took high salt diet as compared to group B who received zinc supplementation in addition to salt
Conclusion: High salt diet exerted harmful effects on bones due to increased sodium chloride induced hypercalciuria leading to bone loss. Zinc is valuable in ameliorating the detrimental effects of salt on bones by enhancing osteoblast activity and inhibiting bone resorbing cells